Using a combination of chitosan, methyl jasmonate, and cyclodextrin as an effective elicitation strategy for prenylated stilbene compound production in Arachis hypogaea L. hairy root culture and their impact on genomic DNA

Peanut (Arachis hypogaea L.) hairy roots are a potential tool for stilbene compound production. This study focuses on the most efficient elicitation strategy inducing production, especially prenylated forms of trans-arachidin-1 and trans-arachidin-3. To best our knowledge, this is first report simultaneous treatment involving chitosan (CHT), methyl jasmonate (MeJA), cyclodextrin (CD); CHT + MeJA CD in peanut to induce substantial amounts trans-arachidin-3 at 72 h period. The results demonstrate highest trans-arachidin-3, with 684.30 ± 183.85 543.94 171.17 mg/g dry weight roots, respectively. antioxidant capacity determined by ABTS FRAP assays was 188.95 0.43 µmol Trolox/g 24.61 0.50 mg ascorbic acid/g respectively, while phenolic content also detected crude extract, 8.67 0.11 gallic roots. pro-oxidant activity extract protection damage DNA great interest can have major positive impacts health promotion disease prevention. Chitosan, jasmonate, high compounds. could scavenge free radicals protect DNA, causes severe genomic DNA.